<!DOCTYPE html>
<html class="client-nojs vector-feature-language-in-header-enabled vector-feature-language-in-main-page-header-disabled vector-feature-page-tools-pinned-disabled vector-feature-toc-pinned-clientpref-0 vector-toc-not-available vector-feature-main-menu-pinned-disabled vector-feature-limited-width-clientpref-1 vector-feature-limited-width-content-enabled vector-feature-custom-font-size-clientpref-1 vector-feature-appearance-pinned-clientpref-0 skin-theme-clientpref-day vector-sticky-header-enabled" lang="de" dir="ltr"><head>
<meta charset="UTF-8">
<title>Split-TEV</title>
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<link rel="icon" type="image/png" href="./_res_/favicon.png">
<link rel="canonical" href="https://de.wikipedia.org/wiki/Split-TEV"> <link href="./_mw_/ext.cite.styles.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.wikimediamessages.styles.css" rel="stylesheet" type="text/css">
<link href="./_mw_/skins.vector.icons.css" rel="stylesheet" type="text/css">
<link href="./_mw_/skins.vector.search.codex.styles.css" rel="stylesheet" type="text/css">
<link href="./_mw_/skins.vector.styles.css" rel="stylesheet" type="text/css">
<meta name="ResourceLoaderDynamicStyles" content="">
<link href="./_mw_/ext.gadget.citeRef.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.defaultPlainlinks.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.dewikiCommonHide.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.dewikiCommonLayout.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.dewikiCommonStyle.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.dewikiDarkmode.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.dewikiResponsive.css" rel="stylesheet" type="text/css">
<link href="./_mw_/ext.gadget.specialSearch.css" rel="stylesheet" type="text/css">
<link rel="stylesheet" type="text/css" href="./_mw_/site.styles.css">
<link rel="stylesheet" type="text/css" href="./_mw_/noscript.css">
<link rel="stylesheet" type="text/css" href="./_res_/footer.css">
<link rel="stylesheet" type="text/css" href="./_res_/vector-2022.css">
</head>
<body class="skin--responsive skin-vector skin-vector-search-vue mediawiki ltr sitedir-ltr mw-hide-empty-elt ns-0 ns-subject page-Split-TEV rootpage-Split-TEV skin-vector-2022 action-view">
<div class="mw-page-container">
<div class="mw-page-container-inner">
<div class="mw-content-container">
<main id="content" class="mw-body">
<header class="mw-body-header vector-page-titlebar">
<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Split-TEV</span></h1>
</header>
<a id="top"></a>
<div id="bodyContent" class="vector-body ve-init-mw-desktopArticleTarget-targetContainer" aria-labelledby="firstHeading" data-mw-ve-target-container="">
<div id="contentSub">
<div id="mw-content-subtitle"></div>
</div>
<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p>Das <b>Split-TEV</b> (‚Gespaltenes TEV‘) ist eine biochemische Methode zum Nachweis von <a href="Protein-Protein-Interaktion" title="Protein-Protein-Interaktion">Protein-Protein-Interaktionen</a> in lebenden Zellen.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>
</p>
<div class="mw-heading mw-heading2"><h2 id="Prinzip">Prinzip</h2></div>
<p>Das Split-TEV ist als <a href="Protein-fragment_complementation_assay" class="mw-redirect" title="Protein-fragment complementation assay">Protein-fragment complementation assay</a> eine Methode zum Nachweis einer Bindung durch Komplementation von Fragmenten eines <a href="Reporterprotein" class="mw-redirect" title="Reporterprotein">Reporterproteins</a> (<a href="Englische_Sprache" title="Englische Sprache">englisch</a> <i>protein complementation assay</i>, PCA), wie auch die <a href="Bimolekulare_Fluoreszenzkomplementation" title="Bimolekulare Fluoreszenzkomplementation">bimolekulare Fluoreszenzkomplementation</a>. Das Split-TEV besteht aus einer NIa-<a href="Protease" class="mw-redirect" title="Protease">Protease</a> des <i>Tobacco Etch Virus</i> (TEV-Protease), die in zwei <a href="Enzym" title="Enzym">enzymatisch</a> inaktive Teile zerlegt wurde. Je ein Teil (das <a href="N-Terminus" title="N-Terminus"><i>N</i>-terminale</a> NTEV oder das <a href="C-Terminus" title="C-Terminus"><i>C</i>-terminale</a> CTEV) wird an die beiden zu untersuchenden <a href="Protein" title="Protein">Proteine</a> als <a href="Fusionsprotein" title="Fusionsprotein">Fusionsprotein</a> angehängt, wodurch bei einer Bindung der beiden Proteine die angehängten Protease-Teile rekonstituiert und die Protease enzymatisch aktiv wird.
</p>
<div class="mw-heading mw-heading2"><h2 id="Literatur">Literatur</h2></div>
<ul><li>Michael C Wehr, Lisa Reinecke, Anna Botvinnik, Moritz J Rossner: <cite style="font-style:italic">Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV</cite>. In: <cite style="font-style:italic">BMC Biotechnology</cite>. 8. Jahrgang, 2008, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>55</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1186/1472-6750-8-55">10.1186/1472-6750-8-55</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/18620601?dopt=Abstract">PMID 18620601</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483975/">PMC 2483975</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Split-TEV&rft.atitle=Analysis+of+transient+phosphorylation-dependent+protein-protein+interactions+in+living+mammalian+cells+using+split-TEV&rft.au=Michael+C%26%2332%3BWehr%2C%26%2332%3BLisa%26%2332%3BReinecke%2C%26%2332%3BAnna%26%2332%3BBotvinnik%2C+...&rft.btitle=BMC+Biotechnology&rft.date=2008&rft.doi=10.1186%2F1472-6750-8-55&rft.genre=book&rft.pages=55&rft.pmc=2483975&rft.pmid=18620601&rft.volume=8.+Jahrgang" style="display:none"> </span></li>
<li>Minou S. Djannatian, Sabrina Galinski, Tobias M. Fischer, Moritz J. Rossner: <cite style="font-style:italic">Studying G protein-coupled receptor activation using split-tobacco etch virus assays</cite>. In: <cite style="font-style:italic">Analytical Biochemistry</cite>. 412. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>2</span>, 2011, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>141–52</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1016/j.ab.2011.01.042">10.1016/j.ab.2011.01.042</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/21295005?dopt=Abstract">PMID 21295005</a>.<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:Split-TEV&rft.atitle=Studying+G+protein-coupled+receptor+activation+using+split-tobacco+etch+virus+assays&rft.au=Minou+S.%26%2332%3BDjannatian%2C%26%2332%3BSabrina%26%2332%3BGalinski%2C%26%2332%3BTobias+M.%26%2332%3BFischer%2C+...&rft.date=2011&rft.doi=10.1016%2Fj.ab.2011.01.042&rft.genre=journal&rft.issue=2&rft.jtitle=Analytical+Biochemistry&rft.pages=141-52&rft.pmid=21295005&rft.volume=412.+Jahrgang" style="display:none"> </span></li></ul>
<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-1"><span class="mw-cite-backlink"><a href="#cite_ref-1">↑</a></span> <span class="reference-text">M. C. Wehr, R. Laage, U. Bolz, T. M. Fischer, S. Grünewald, S. Scheek, A. Bach, K. A. Nave, M. J. Rossner: <i>Monitoring regulated protein-protein interactions using split TEV.</i> In: <i>Nature methods.</i> Band 3, Nummer 12, Dezember 2006, S. 985–993, <span class="-print"><a href="Internationale_Standardnummer_f%C3%BCr_fortlaufende_Sammelwerke" title="Internationale Standardnummer für fortlaufende Sammelwerke">ISSN</a> <span style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://zdb-katalog.de/list.xhtml?t=iss%3D%221548-7091%22&key=cql">1548-7091</a></span></span>. <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1038/nmeth967">10.1038/nmeth967</a></span>. <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/17072307?dopt=Abstract">PMID 17072307</a>.</span>
</li>
</ol></div><!--htdig_noindex--><div><div class="zim-footer">
Dieser Artikel wurde von <a class="external text" title="Zuletzt bearbeitet am 2014-10-07" href="https://de.wikipedia.org/wiki/?title=Split-TEV&oldid=134687371">Wikipedia</a> herausgegeben. Der Text ist unter <a class="external text" href="https://creativecommons.org/licenses/by-sa/4.0/deed.de">Creative Commons Attribution-Share Alike 4.0</a> verfügbar, sofern nicht anders angegeben. Für die Mediendateien können zusätzliche Bedingungen gelten.
</div>
</div><!--/htdig_noindex--></div>
</div>
</main>
</div>
</div>
</div>
<script src="./_webp_/webpHandler.js"></script>
</body></html>